Combined RNAseq and ChIPseq Analyses of the BvgA Virulence Regulator of Bordetella pertussis
نویسندگان
چکیده
منابع مشابه
Virulence Factors Variation Among Bordetella Pertussis Isolates in Iran
Pertussis is still endemic and the recently resurgence of the disease caused by Bordetella pertussis has been shown in many countries. The polymorphism of the virulence genes of B. pertussis and lack of any information about the allelic variation between the Iranian isolates promotes us to analysis of the genes encoded virulence factors including ptxS1, prn, fim3 and cya to understand the diffe...
متن کاملGenetic and biochemical analyses of BvgA interaction with the secondary binding region of the fha promoter of Bordetella pertussis.
The BvgA-BvgS two-component signal transduction system regulates expression of virulence factors in Bordetella pertussis. The BvgA response regulator activates transcription by binding to target promoters, which include those for the genes encoding filamentous hemagglutinin (fha) and pertussis toxin (ptx). We have previously shown that at both promoters the phosphorylated form of BvgA binds mul...
متن کاملSeparation and Detection of Phosphorylated and Nonphosphorylated BvgA, a Bordetella pertussis Response Regulator, in vivo and in vitro.
Protein phosphorylation plays a central role in signal transduction in bacteria. However, separation and detection of the phosphorylated protein from its nonphosphorylated form remain challenging. Here we describe a method to detect phosphorylation of the Bordetella pertussis response regulator BvgA, which is phosphorylated at an aspartate residue (Boulanger et al., 2013). This method is based ...
متن کاملCharacterization of DNA binding sites for the BvgA protein of Bordetella pertussis.
Expression of virulence-associated genes in Bordetella pertussis is under the control of the pleiotropic regulator BvgA. Although previous studies have identified recognition sequences for BvgA in several promoter regions, their structures have not been clearly characterized. We show that the BvgA binding sites within the bvgp(1) and cyaA promoters consist of inverted repeats and suggest that i...
متن کاملRegulation of pertussis toxin and lipopolysaccharide levels of Bordetella pertussis 134 in response to modulators
Whooping cough (pertussis) is a highly contagious disease of the human respiratory tract, which is caused by Bordetella pertussis. Reemerge of pertussis in some highly immunized populations and divergency in gene order among several B. pertussis strains promoted this research to study the change of pertussis toxin (PT) and lipopolysacharide levels in response to the different environments. Th...
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ژورنال
عنوان ژورنال: mSystems
سال: 2020
ISSN: 2379-5077
DOI: 10.1128/msystems.00208-20